Transcription profiling of rat adrenal medulla and PC12 cell culture to investigate genetic networks regulated by short chain fatty acids

Genome-wide expression profiling showed significant overlap of the genetic networks regulated by butyrate in vivo (rat adrenal medulla) and in vitro (PC12 cells) including TH and other neurotransmitter-related genes (DBH, AADC, GTPCH, ppEnk, NPY). Experiment Overall Design: Studies in PC12 cells were performed to delineate which genetic networks are regulated by SCFA depending on the dose. Experiments in vivo were performed to test wheter similar phenomenon operates in the sympathoadrenal system. Experiment Overall Design: ISBPC: 1mMSB PC12 Experiment Overall Design: GSBPC: 6mMSB PC12 Experiment Overall Design: CPCR: CPC12 Experiment Overall Design: SBAM: rat SBAdrenal Medulla Experiment Overall Design: CAM: rat Control Adrenal Medulla