Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic progenitors co-occupied with lineage specific regulators (GATA1, GATA2)

·发布 2011年10月7日 ·更新 2013年4月3日

样本数

实验数

相关文献

实验描述

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human CD34 ChIP-seq portion of this dataset. Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008) or CEBPA (SC-9314).

参考文献

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration.

Trompouki E, Bowman TV, Lawton LN, Fan ZP, Wu DC, DiBiase A, Martin CS, Cech JN, Sessa AK, Leblanc JL, Li P, Durand EM, Mosimann C, Heffner GC, Daley GQ, Paulson RF, Young RA, Zon LI

PMID: 22036566

样本属性

antibody catalog number

None WCE, Santa Cruz SC-265, Santa Cruz SC-7965, Santa Cruz SC-8631, Santa Cruz SC-9008

cell type

Hematopoietic Stem & Progenitor Cells, Pro-erythroblasts

chip antibody

GATA1, GATA2, SMAD1, TCF7L2, WCE

Organism

Homo sapiens

实验信息

登记号

E-GEOD-29194

GEO 编号

SRP008214, GSE29194

实验类型

ChIP-seq

物种

Homo sapiens

发布日期

2011年10月7日

更新日期

2013年4月3日

提交者

L N Lawton、 Z P Fan、 R A Young、 G C Heffner、 P Li、 E Trompouki、 R F Paulson、 T V Bowman、 G Q Daley、 Teresa Venezia Bowman、 L I Zon、 E M Durand、 A DiBiase、 C S Martin、 A K Sessa、 C Mosimann、 J N Cech、 D Wu、 J L Leblanc

外部链接

ArrayExpress 原始页 NCBI GEO：SRP008214, GSE29194

分析服务

Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic progenitors co-occupied with lineage specific regulators (GATA1, GATA2)

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