Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)

·发布 2011年10月7日 ·更新 2014年5月2日

样本数

实验数

相关文献

实验描述

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human ChIP-seq portion of this dataset on leukemia cell lines. Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008), CEBPA (SC-9314) or H3K4me1 (Abcam ab8895).

参考文献

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration.

Trompouki E, Bowman TV, Lawton LN, Fan ZP, Wu DC, DiBiase A, Martin CS, Cech JN, Sessa AK, Leblanc JL, Li P, Durand EM, Mosimann C, Heffner GC, Daley GQ, Paulson RF, Young RA, Zon LI

PMID: 22036566

样本属性

antibody catalog number

None, None WCE, Santa Cruz SC-265, Santa Cruz SC-6031, Santa Cruz SC-7965, Santa Cruz SC-8631, Santa Cruz SC-9008, Santa Cruz SC-9314

cell line

K562, K562-C/EBPA, U937

cell type

None, K562, K562-C/EBPA

chip antibody

CEBPA, GATA1, GATA2, H3K4me1, SMAD1, TCF7L2, WCE

chip antibody vendor

None, Santa Cruz

Organism

Homo sapiens

实验信息

登记号

E-GEOD-29195

GEO 编号

SRP008215, GSE29195

实验类型

ChIP-seq

物种

Homo sapiens

发布日期

2011年10月7日

更新日期

2014年5月2日

提交者

E Trompouki、 J N Cech、 A DiBiase、 C S Martin、 Teresa Venezia Bowman、 E M Durand、 L N Lawton、 R F Paulson、 G Q Daley、 D Wu、 L I Zon、 R A Young、 A K Sessa、 G C Heffner、 P Li、 C Mosimann、 T V Bowman、 Z P Fan、 J L Leblanc

外部链接

ArrayExpress 原始页 NCBI GEO：SRP008215, GSE29195

分析服务

Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)

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