The role of HIF in FH-associated renal neoplasia

·发布 2011年10月23日 ·更新 2011年11月14日

样本数

实验数

芯片平台

相关文献

实验描述

Fumarate hydratase (FH) mutations predispose to renal cysts cancer. These cancers overexpress hypoxia-inducible factor-alpha (Hif-1a). We have generated a conditional Fh1 (mouse FH) knockout mice that develop renal cysts and overexpress Hif-1a. In order to identify the contribution of Hif-1a to cyst formation we have intercrossed our mice with conditional HIf-1a KO mice. We intercrossed Fh1/Hif1a mice with kidney specific cre recombinase (Ksp-Cre) and analysed kidney cyst formation. RNA was extracted from cysts from 4xFh1 KO, 4xFh1/Hif-1a KO and 4 control mice. For each comparison littermates were used and the animals were aged 15 weeks i.e. early cystic disease.

参考文献

Renal Cyst Formation in Fh1-Deficient Mice Is Independent of the Hif/Phd Pathway: Roles for Fumarate in KEAP1 Succination and Nrf2 Signaling.

Adam J, Hatipoglu E, O'Flaherty L, Ternette N, Sahgal N, Lockstone H, Baban D, Nye E, Stamp GW, Wolhuter K, Stevens M, Fischer R, Carmeliet P, Maxwell PH, Pugh CW, Frizzell N, Soga T, Kessler BM, El-Bahrawy M, Ratcliffe PJ, Pollard PJ

PMID: 22014577

芯片平台

A-MEXP-1175

Illumina MouseWG-6 v2.0 Expression BeadChip（12 例）

样本属性

age

15 weeks

genotype/variation

control, Fh1 KO, Hif1a/Fh1 KO

Organism

Mus musculus

strain

C57BL/6

实验信息

登记号

E-GEOD-29988

GEO 编号

GSE29988

实验类型

transcription profiling by array

物种

Mus musculus

发布日期

2011年10月23日

更新日期

2011年11月14日

提交者

Patrick Pollard、 Helen Lockstone、 Mona El-Bahrawy、 Natasha Sahgal、 Peter Ratcliffe、 Gordon Stamp、 Norma Frizzell、 Emma Nye、 Dilair Baban、 Linda O'Flaherty、 Natasha Sahgal、 Julie Adam、 Chris Pugh、 Emine Hatipoglu、 Benedikt Kessler、 Nicola Ternette

外部链接

ArrayExpress 原始页 NCBI GEO：GSE29988

分析服务

The role of HIF in FH-associated renal neoplasia

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