Gene expression profile of ATF6a and ATF6b knockout mice with dextran sulfate sodium (DSS)-induced colitis

·发布 2015年2月8日 ·更新 2015年2月14日

样本数

实验数

芯片平台

实验描述

To understand the role of ATF6a and ATF6b in non-canonical endoplasmic reticulum stress response pathway, we have employed whole genome microarray expression profiling to identify the genes regulated by ATF6s. WIild type or, ER stress mediators,ATF6a or ATF6b-knockout mice were treated with 3% DSS for 3days. Genes responsible for DSS in each mediator-dependent manner were extracted and categorized by Gene Ontology. Among them, expression of three cell structure-related genes (Muc2, Muc3, Muc4) was quantified in the RNA samples from another mice treated with DSS by real-time PCR. Colitis was induced with 3% DSS, 36,000-50,000 M.W. (MP Biomedicals,) in the drinking water for 3 days. Genes responsible for DSS treatment in each mediator-dependent manner were extracted and categorized by Gene Ontology in GeneRanker program of Genomatix platform.

芯片平台

A-MEXP-724

Agilent Whole Mouse Genome Microarray 4x44K 014868 G4122F (annotation from 02.2007)（12 例）

样本属性

genotype

ATF6aKO, ATF6bKO, WT

organism

Mus musculus

organism part

colon

sex

male

strain

C57BL/6J

实验信息

登记号

E-GEOD-35651

GEO 编号

GSE35651

实验类型

transcription profiling by array

物种

Mus musculus

发布日期

2015年2月8日

更新日期

2015年2月14日

提交者

Kiyoe Kurahashi、 Miho Oyadomari、 Taiji Ito、 Mari Funahashi、 Akitoshi Nomura、 Naoko Miura、 Masato Miyake、 Seiichi Oyadomari、 Eiji Tanaka、 Kazue Tsugawa、 Seiko Kagawa、 Robert Zeng、 Kazutoshi Mori、 Chinobu Miyamoto、 Kazuna Takahara、 Seiichi Oyadomari、 Ryousuke Sato、 Tomoko Mori

外部链接

ArrayExpress 原始页 NCBI GEO：GSE35651

分析服务

Gene expression profile of ATF6a and ATF6b knockout mice with dextran sulfate sodium (DSS)-induced colitis

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