Transcriptional profiling of 2D colonies derived from WT, Bmi1-/-, and Bmi1-/-;Ink4a/Arf Labial cervical loops

This experiment is aimed at determining the genetic signature of dental stem cell colonies grown in vitro and how that signature changes when Bmi1 activity is removed. Another question to be answered by the experiment is whether Bmi1 has alternate targets besides Ink4a/Arf. There are 12 total samples: 4 biological replicates for each genotype that consist of single colonies grown in vitro.