E-GEOD-49927
GSE49927
ChIP-chip by tiling array
Saccharomyces cerevisiae
Genome-wide effects of Spt6 on RNA pol II and Histone H3 occupancy in budding yeast (ChIP-Chip)
·Released Aug. 16, 2013
·Updated June 3, 2014
10
Samples
5
Assays
1
Array Platforms
Description
whole genome analysis of RNA pol II and histone H3 in WT and Spt6-depleted cells using a tetracycline regulated ts degron mutant, spt6-td. ChiP-ChIP using ligation-mediated PCR amplified material hybridized to Nimblegen 385K arrays 50mers median probe spacing 32 bp cat. No. C4214-00-01
Array Platforms
A-GEOD-4613
NimbleGen Saccharomyces cerevisiae_ChIP whole-genome tiling array（5 items）
Sample Attributes
chip antibody
12CA5 anti-HA (Roche), input DNA, rabbit anti pan CTD (Schroeder eta l Genes Dev. 14:2435, 2000), rabbit anti-H3 C-terminus (Zhang et al EMBO J. 24:2379, 2005)
organism
Saccharomyces cerevisiae
strain or line
DBY311 (W303 background), DBY311 (W303), DBY875 spt6-td-HA3 (W303), DBY875 spt6-td-HA3 (W303)
Experiment Info
Accession
E-GEOD-49927
GEO ID
GSE49927
Type
ChIP-chip by tiling array
Organism
Saccharomyces cerevisiae
Released
Aug. 16, 2013
Updated
June 3, 2014
Submitter
David L. Bentley、
David Benltey、
Hyunmin Kim
Analysis Services
Contact
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