Examining efficiency of enrichment of kidney pericyte-specific messages by TRAP (Translating Ribosome Affinity Purification)

·发布 2014年3月1日 ·更新 2014年6月3日

样本数

实验数

芯片平台

实验描述

The long term goal is to define the transcriptional changes that accompany pericyte-to-myofibroblast transition in fibrotic kidney disease. Medullary pericytes are identified by their expression of a eGFPL10a fusion protein whose expression is driven by a Col1a1 promoter. Pericyte-specific RNA is generated by eGFP-affinity purification of polysomes from medullary lysates and then subject to microarray analysis. Col1a1-eGFPL10a mice were subject to Sham or unilateral ureteral obstruction surgery. Sham kidneys were collected at day 0, and UUO kidneys were collected at day 2 or day 5 for TRAP.

芯片平台

A-AFFY-45

Affymetrix GeneChip Mouse Genome 430 2.0 [Mouse430_2]（15 例）

样本属性

developmental stage

P14-P300

experimental design

Col1a1-eGFPL10a mice were subject to Sham or unilateral ureteral obstruction surgery. Sham kidneys were collected at day 0, and UUO kidneys were collected at day 2 or day 5 for TRAP.

mutation notes

Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element.

organism

Mus musculus

organism part

kidney medulla, kidney pericytes (periarterial fibroblasts of inner and outer medulla)

Sex

both

specimen mutation

Tg:Col1a1-eGFPL10a

strain

C57/129sv

thelier stage

url

http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21179, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21180, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21181, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21182, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21183, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21184, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21185, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21186, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21187, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21188, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21189, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21190, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21191, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21192, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21193

实验信息

登记号

E-GEOD-50439

GEO 编号

GSE50439

实验类型

transcription profiling by array

物种

Mus musculus

发布日期

2014年3月1日

更新日期

2014年6月3日

提交者

GUDMAP Developers、 Ben D Humphreys

外部链接

ArrayExpress 原始页 NCBI GEO：GSE50439

分析服务

Examining efficiency of enrichment of kidney pericyte-specific messages by TRAP (Translating Ribosome Affinity Purification)

联系地址

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