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E-GEOD-53156 GSE53156 transcription profiling by array Mus musculus

Gene expression profiles of wild type and Actinin 4 point mutant knock-in adult podocyte cells at 2, 5 and 44 weeks following TRAP purification. (GUDMAP Series ID: 49)

·发布 2014年2月1日 ·更新 2014年6月3日
25
样本数
25
实验数
1
芯片平台
实验描述

The long term goal is to create an encyclopaedia of podocyte gene expression both in homeostasis, development and disease. Podocytes are identified by their expression of an eGFPL10a fusion protein whose expression is driven by a Col1a1 promoter fragment. Podocyte-specific RNA is generated by eGFP-affinity purification of polysomes and then subject to microarray analysis. Expression profiling of wild type and Actinin 4 point mutant knock-in kidney podocytes by TRAP. Col1a1-eGFPL10a mice were crossed against Actn4+/tm1.1Plk (KI) mice. Wild-type or KI mice were subject to TRAP at the following time points: 2, 5 and 44 weeks.

芯片平台
A-GEOD-11180
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate(25 例)
样本属性
cell type
kidney podocytes (visceral epithelium of mature renal corpuscle, synonym: podocyte layer)
developmental stage
P14
experimental design
Col1a1-eGFPL10a mice were crossed against Actn4+/tm1.1Plk (KI) mice. Wild-type or KI mice were subject to TRAP at the following time points: 2, 5 and 44 weeks.
mutation notes
Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element., Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208719, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208720, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208721, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208722, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208723, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208724, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208725, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208726, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208727, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208728, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208729, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208730
organism
Mus musculus
organism part
kidney podocytes, P14, kidney podocytes, P300, kidney podocytes, P35
Sex
unknown
specimen mutation
Tg:Col1a1-eGFPL10a, Tg:Col1a1-eGFPL10a and Actn4tm1.1Plk (MGI:3767564)
strain
C57/129sv
thelier stage
28
url
http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21085, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21086, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21087, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21088, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21089, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21090, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21091, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21092, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21093, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21094, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21095, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21096, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21097, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21098, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21099, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21100, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21101, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21102, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21103, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21104, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21105, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21106, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21107, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21108, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21109
实验信息
登记号
E-GEOD-53156
GEO 编号
GSE53156
实验类型
transcription profiling by array
物种
Mus musculus
发布日期
2014年2月1日
更新日期
2014年6月3日
提交者
Ben D Humphreys、 GUDMAP Developers
分析服务
分析服务

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