实验描述
The long term goal is to create an encyclopaedia of podocyte gene expression both in homeostasis, development and disease. Podocytes are identified by their expression of an eGFPL10a fusion protein whose expression is driven by a Col1a1 promoter fragment. Podocyte-specific RNA is generated by eGFP-affinity purification of polysomes and then subject to microarray analysis. Expression profiling of wild type and Actinin 4 point mutant knock-in kidney podocytes by TRAP. Col1a1-eGFPL10a mice were crossed against Actn4+/tm1.1Plk (KI) mice. Wild-type or KI mice were subject to TRAP at the following time points: 2, 5 and 44 weeks.
样本属性
cell type
kidney podocytes (visceral epithelium of mature renal corpuscle, synonym: podocyte layer)
experimental design
Col1a1-eGFPL10a mice were crossed against Actn4+/tm1.1Plk (KI) mice. Wild-type or KI mice were subject to TRAP at the following time points: 2, 5 and 44 weeks.
mutation notes
Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element., Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208719, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208720, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208721, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208722, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208723, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208724, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208725, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208726, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208727, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208728, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208729, Construct generated in Ben Humphreys lab. A fusion transgene encoding eGFP and the ribosomal protein L10a was inserted downstream of the collagen1α1 promoter/enhancer element. Actn4tm1.1Plk - PMID:15208730
organism part
kidney podocytes, P14, kidney podocytes, P300, kidney podocytes, P35
specimen mutation
Tg:Col1a1-eGFPL10a, Tg:Col1a1-eGFPL10a and Actn4tm1.1Plk (MGI:3767564)
url
http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21085, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21086, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21087, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21088, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21089, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21090, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21091, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21092, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21093, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21094, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21095, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21096, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21097, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21098, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21099, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21100, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21101, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21102, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21103, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21104, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21105, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21106, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21107, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21108, http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:21109