RNA-seq of mouse ES cells with conditional Drosha knockout against wild type controls to identify pri-miRNAs

·Released Feb. 9, 2015 ·Updated May 1, 2015

Samples

Assays

References

Description

We report the ability of the Drosha null/conditional-null mouse model to enable the identification of pri-miRNA transcripts. The conditional-null allele of Drosha phenocopies the null allele both in mESC and in mice, upon conversion to the null state with Cre. Examination of the effects of Drosha deficiency in mouse embryonic stem cells.

References

microTSS: accurate microRNA transcription start site identification reveals a significant number of divergent pri-miRNAs.

Georgakilas G, Vlachos IS, Paraskevopoulou MD, Yang P, Zhang Y, Economides AN, Hatzigeorgiou AG

PMID: 25492647

Sample Attributes

cell type

embryonic stem cell

genotype

Drosha -/-, wild type genotype

organism

Mus musculus

strain

C57BL/6

Experiment Info

Accession

E-GEOD-55735

GEO ID

GSE55735, SRP039604

Type

RNA-seq of coding RNA

Organism

Mus musculus

Released

Feb. 9, 2015

Updated

May 1, 2015

Submitter

Artemis Hatzigeorgiou、 Peter Yang、 Aris Economides

External Links

ArrayExpress source NCBI GEO：GSE55735, SRP039604

Analysis Services

RNA-seq of mouse ES cells with conditional Drosha knockout against wild type controls to identify pri-miRNAs

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