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E-GEOD-74346 GSE74346 transcription profiling by array Arabidopsis thaliana

Expression data from Arabidopsis wild-type, KO-nudx6, KO-nudx7, and double KO-nudx6/7 plants

·Released Oct. 28, 2015 ·Updated Dec. 8, 2015
8
Samples
8
Assays
1
Array Platforms
Description

Arabidopsis Nudix hydrolases, AtNUDX6 and 7, exhibit pyrophosphohydrolase activities toward NADH and contribute to the modulation of various defense responses, such as the poly(ADP-ribosyl)ation (PAR) reaction and salicylic acid (SA)-induced Nonexpresser of Pathogenesis-Related genes 1 (NPR1)-dependent defense pathway, against biotic and abiotic stresses. To identify genes (NRGs) whose expression levels positively and negatively correlated with NADH levels, a transcriptome analysis using KO-nudx6, KO-nudx7, and double KO-nudx6/7 plants, in which intracellular NADH levels increased in a step-wise manner. Arabidopsis wild-type, KO-nudx6, KO-nudx7, and double KO-nudx6/7 plants were grown on MS medium in a growth chamber kept at 23°C during 16 h of light (100 µmol/m2/s) and at 22°C during 8 h of darkness. Two-week-old cotyledons were collected as samples for microarray analysis.

Array Platforms
A-AFFY-2
Affymetrix GeneChip Arabidopsis Genome [ATH1-121501](8 items)
Sample Attributes
age
two weeks
ecotype
Col-0
genotype
KO-nudx6, KO-nudx6/7, KO-nudx7, wild type
organism
Arabidopsis thaliana
organism part
cotyledons
Experiment Info
Accession
E-GEOD-74346
GEO ID
GSE74346
Type
transcription profiling by array
Organism
Arabidopsis thaliana
Released
Oct. 28, 2015
Updated
Dec. 8, 2015
Submitter
Shigeru Shigeoka、 Takahisa Ogawa、 Kazuya Yoshimura
Analysis Services
Analysis Services

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