Transcription profiling by array of human neuroectoderm or embryonic stem cells after Pax6 knockdown

Stable Pax6 and Luciferase knockdown human embryonic stem cell lines (H9) were made through lentiviral infection. Two different Pax6 RNAi lines and two Luciferase RNAi lines were then differentiated to neuroectoderm cells for 6 days. mRNA pooled from these two individual lines were then subjected to gene expression profiling analysis using affymetrix U133 plus 2.0 array.