Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer

·发布 2021年4月8日

120

样本数

120

实验数

相关文献

实验描述

PBMCs from 4 donors were stained with the influenza A virus HLA-A24/PB1 498-505 tetramer. Two donors (non-LIFT8 and non-LIFT12) showed TCR independent KIR binding to tetramers whereas the other two donors (Non-LIFT14 and Non-LIFT10) showed TCR specific binding. Cells were single-cell sorted on a BD Aria III into 96-well plates containing 0.5 μl dNTP mix (10 mM), 0.5 μl oligo-dT pri- mer (5 μM), and 1 μl lysis buffer (prepared by adding 1 μl RNase inhibitor to 19 μl Triton X-100 solution, 0.2% v/v).

参考文献

CD8+ T-cell landscape in Indigenous and non-Indigenous people restricted by influenza mortality-associated HLA-A*24:02 allomorph

Luca Hensen, Patricia T. Illing, E. Bridie Clemens, Thi H.O. Nguyen, Marios Koutsakos, Carolien E. van de Sandt, Nicole A. Mifsud, Andrea Nguyen, Christopher Szeto, Brendon Y. Chua, Hanim Halim, Simone Rizzetto, Fabio Luciani, Liyen Loh, Emma J. Grant, Phillipa M. Saunders, Andrew G Brooks, Steve Rockman, Tom C. Kotsimbos, Allen C. Cheng, Michael Richards, Glen P. Westall, Linda M. Wakim, Thomas Loudovaris, Stuart I. Mannering, Michael Elliott, Stuart G. Tangye, David C Jackson, Katie L Flanagan, Jamie Rossjohn, Stephanie Gras, Jane Davies, Adrian Miller, ProfileSteven Y.C. Tong, Anthony W. Purcell, Katherine Kedzierska

样本属性

Organism

Homo sapiens

Developmental stage

adult

Disease

normal

Organism part

blood

Cell type

CD8-positive, alpha-beta T cell

Genotype

wild type genotype

Phenotype

PB1 498-505+

Individual

Non-Lift 10, Non-Lift 14, Non-Lift 12, Non-Lift 8

Age

59 year, 38 year, 24 year, 34 year

Sex

male, female

实验信息

登记号

E-MTAB-10109

实验类型

RNA-seq of coding RNA from single cells, Human - Single-cell sequencing

物种

Homo sapiens, Homo sapiens

发布日期

2021年4月8日

提交者

Katherine Kedzierska

外部链接

ArrayExpress 原始页

分析服务

Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer

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