实验描述
Five hiPSC lines from four individuals were differentiated to neural progenitor cells. The two cell types were compared in regards to marker gene expression of known cell type markers to show that the used bulk RNA-seq protocol, called prime-seq can capture known biological differences.
参考文献
prime-seq, a simple, inexpensive, and powerful bulk RNA-sequencing protocol for detecting differential expression
Aleksandar Janjic, Lucas E. Wange, Johannes W. Bagnoli, Johanna Geuder, Phong Nguyen, Daniel Richter, Beate Vieth, Christoph Ziegenhain, Binje Vick, Ines Hellmann, Wolfgang Enard
样本属性
Genotype
wild type genotype
Progenitor cell type
induced pluripotent stem cell
Individual
Human5, Human1, Human2, Human3, Human4
Cell type
induced pluripotent stem cell, neural progenitor cell
Cell line
12C2, 1383B2, 63AB2.2, ... 7 other values
Sample barcode
CAGCGGAACTTA, CATCAACTCGAA, CCTAACCTACAA, ... 7 other values