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E-MTAB-10169 RNA-seq of coding RNA from single cel... Homo sapiens, Homo sapiens

A single-cell atlas of lymphocyte adaptive immune repertoires and transcriptomes reveals age-related differences in convalescent COVID-19 patients

·发布 2021年3月20日

样本数

实验数

相关文献

实验描述

We utilize single-cell sequencing (scSeq) of lymphocyte immune repertoires and transcriptomes to quantitatively profile the adaptive immune response in COVID-19 patients of varying age. Our scSeq analysis defines the adaptive immune repertoire and transcriptome in convalescent COVID-19 patients and shows important age-related differences implicated in immunity against SARS-CoV-2.

参考文献

A single-cell atlas of lymphocyte adaptive immune repertoires and transcriptomes reveals age-related differences in convalescent COVID-19 patients

Florian Bieberich, Rodrigo Vazquez-Lombardi, Alexander Yermanos, Roy A. Ehling, Derek M. Mason, Bastian Wagner, Edo Kapetanovic, Raphael Brisset Di Roberto, Cédric R. Weber, Miodrag Savic, Fabian Rudolf, Sai T. Reddy

样本属性

Organism

Homo sapiens

Developmental stage

adult

Organism part

blood

ELISA

ELISA: IgA positive; IgG positive

Infect

Severe acute respiratory syndrome coronavirus 2

Individual

individual 4, individual 3, individual 8, individual 6, individual 5, individual 2, individual 7, individual 1

Body mass index

28.7, 24.4, 23.2, 26, 22, 23.4, 28.9, 33.9

Sex

male, female

Age

31 year, 37 year, 63 year, 71 year, 33 year, 23 year, 59 year, 74 year

Immunophenotype

CD138 positive

Cell type

T cell, B cell, mononuclear cell

Sampling timepoint

32 day, 34 day, 30 day, 41 day, 33 day, 39 day

Lateral flow test

lateral flow test: IgM negative; IgG positive, lateral flow test: IgM positive; IgG positive

Clinical information

help needed, no restrictions, bedridden

Iga titer od ratio

IgA titer OD ratio: 6.5, IgA titer OD ratio: 4.1, IgA titer OD ratio: 8.1, IgA titer OD ratio: 13.5, IgA titer OD ratio: 9.9, IgA titer OD ratio: 10.8, IgA titer OD ratio: 7.7, IgA titer OD ratio: 4.8

Igg titer od ratio

IgG titer OD ratio: 12.4, IgG titer OD ratio: 5.7, IgG titer OD ratio: 9.1, IgG titer OD ratio: 3.3, IgG titer OD ratio: 9.5, IgG titer OD ratio: 12.8, IgG titer OD ratio: 13.1, IgG titer OD ratio: 8.5

Clinical history

previous Covid-19 infection for 4 days, previous Covid-19 infection for 9 days, previous Covid-19 infection for 14 days, previous Covid-19 infection for 12 days, previous Covid-19 infection for 7 days

Cohort

older patient group, young patient group

Disease staging

moderate, mild

Protocol

VDJ-TCR enrichment, gene expression, VDJ-BCR enrichment

实验信息

登记号

E-MTAB-10169

实验类型

RNA-seq of coding RNA from single cells, Human - High-throughput sequencing

物种

Homo sapiens, Homo sapiens

发布日期

2021年3月20日

提交者

Florian Bieberich

外部链接

ArrayExpress 原始页

分析服务

A single-cell atlas of lymphocyte adaptive immune repertoires and transcriptomes reveals age-related differences in convalescent COVID-19 patients

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