RNA-Seq analysis of E15.5 mouse Insm1, Neurod1 and Pax6 knockouts vs Insm1GFP.Cre/+ controls

·Released Aug. 23, 2021

Samples

Assays

References

Description

This experiment used RNA-Seq technology to explore gene expression in mouse Insm1GFP.Cre/+ controls and Insm1 (Insm1GFP.Cre/GFP.Cre), Neurod1 (Insm1GFP.Cre/+; Neurod1LacZ/LacZ), or Pax6 (Insm1GFP.Cre/+; Pax6fl/fl) knockout FACS sorted pancreatic endocrine cells at E15.5. Comparison of Insm1GFP.Cre+/- and knockout animals revealed sets of differentially expressed genes that are required for endocrine cell specification and development.

References

Insm1, Neurod1, and Pax6 promote murine pancreatic endocrine cell development through overlapping yet distinct RNA transcription and splicing programs

Dudek KD, Osipovich AB, Cartailler JP, Gu G, Magnuson MA

PMID: 34534285

Sample Attributes

Organism

Mus musculus

Developmental stage

embryo stage

Age

15.5

Organism part

endocrine pancreas

Strain

Mixed CD-1 x C57BL/6J, CD-1

Genotype

Insm1GFP.Cre/GFP.Cre, Insm1GFP.Cre/+; Neurod1LacZ/LacZ, Insm1GFP.Cre/+; Pax6fl/fl, Insm1GFP.Cre/+

Individual

1, 2, 3, ... 9 other values

Experiment Info

Accession

E-MTAB-10262

Type

RNA-seq of coding RNA

Organism

Mus musculus, Mus musculus

Released

Aug. 23, 2021

Submitter

Mark Magnuson、 Karrie Dudek、 Shristi Shrestha、 Jean-Philippe Cartailler

External Links

ArrayExpress source

Analysis Services

RNA-Seq analysis of E15.5 mouse Insm1, Neurod1 and Pax6 knockouts vs Insm1GFP.Cre/+ controls

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