Single-cell RNA-seq of Endometrial organoids treated with NOTCH or WNT inhibitors in a time course of Estrogen and Progesterone stimulation

·发布 2021年7月21日

样本数

实验数

相关文献

实验描述

We performed single-cell RNA-sequencing of 3D endometrial organoid cultures in order to dissect the signaling pathways that determine cell fate of epithelial lineages. Every time course was obtained in a background that either included or did not include ovarian hormones stimulation, which emulates dynamic regulations associated with the menstrual cycle. These inductions show that in vitro downregulation of WNT or NOTCH pathways increases the differentiation efficiency along the secretory and ciliated lineages, respectively.

参考文献

Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro

Luz Garcia-Alonso, Louis-François Handfield, Kenny Roberts, Konstantina Nikolakopoulou, Ridma C. Fernando, Lucy Gardner, Ben Woodhams, Anna Arutyunyan, Krzysztof Polanski, Regina Hoo, Carmen Sancho-Serra, Tong Li, Kwasi Kwakwa, Elizabeth Tuck, Vitalii Kleshchevnikov, Aleksandra Tarkowska, Tarryn Porter, Cecilia Icoresi Mazzeo, Stijn van Dongen, Monika Dabrowska, Vasyl Vaskivskyi, Krishna T. Mahbubani, Jong-eun Park, Mercedes Jimenez-Linan, Lia Campos, Vladimir Kiselev, Cecilia Lindskog, Paul Ayuk, Elena Prigmore, Michael R Stratton, Kourosh Saeb-Parsy, Ashley Moffett, Luiza Moore, Omer A. Bayraktar, Sarah A. Teichmann, Margherita Y. Turco, Roser Vento-Tormo

样本属性

Organism

Homo sapiens

Developmental stage

adult

Sex

female

Organism part

endometrium

Cell type

epithelial cell

Growth condition

organoid culture

Stimulus

none, estrogen, estrogen and progesterone

实验信息

登记号

E-MTAB-10283

实验类型

RNA-seq of coding RNA from single cells

物种

Homo sapiens, Homo sapiens

发布日期

2021年7月21日

提交者

Louis-Francois Handfield

外部链接

ArrayExpress 原始页

分析服务

Single-cell RNA-seq of Endometrial organoids treated with NOTCH or WNT inhibitors in a time course of Estrogen and Progesterone stimulation

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