RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS

To identify AP-1 regulated genes in KYAE-1 and OE19 oesophageal adenocarcinoma cells we induced expression of dominant negative (acidic) FOS to inhibit AP-1 activity and then performed RNA-seq. Acidic FOS (aFOS, J Biol Chem. 1997 Jul 25;272(30):18586-94. doi: 10.1074/jbc.272.30.18586) was expressed from the pINDUCER20 vector.