RNA-Seq of human fibroblasts, induced neurons (iNs), iPSCs, and iPSC-derived neurons, and ATAC-Seq of fibroblasts and iNs from a cohort of Alzheimer patients and controls

We have obtained fibroblast cultures from old adult human donors, including Alzheimer patients. The fibroblasts were reprogrammed into directly induced neurons (iNs) to serve as an adult-like and age-equivalent model for aging and neurodegeneration. We also generated iPSCs and rejuvenated iPSC-derived induced neurons from a subset of the same cohort as controls. The cells were analyzed using several assays, including mRNA-Seq, ATAC-Seq and DNA methylation EPIC array analysis.