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E-MTAB-10814 ChIP-seq Homo sapiens, Homo sapiens

DRIP-seq files for: DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci

·发布 2022年11月8日
8
样本数
8
实验数
实验描述

Determining the role of DDX17 in the formation of DNA:RNA-hybrids around active DNA double-strand breaks (DSBs) using DRIP-seq in the damaged induced via AsiSI (DIvA) cell system that induced DSBs at known genomic loci in response to hydroxytamoxifen (OHT) treatment via and AsiSI enzyme fused to an oestrogen receptor. Sequencing was done using either control or DDX17 siRNA, and mock or 4 hours 300nM OHT treatment. Paired-end 150 cycles was completed on an Illumina NextSeq 500 and library prep was completed using the NEB NEBNext Ultra II library prep kit.

样本属性
Organism
Homo sapiens
Developmental stage
na
Disease
osteosarcoma
Genotype
wild type genotype
Organism part
na
Cell type
epithelial cell
Cell line
U2OS
Replicate
1, 2
实验信息
登记号
E-MTAB-10814
实验类型
ChIP-seq
物种
Homo sapiens, Homo sapiens
发布日期
2022年11月8日
提交者
Aldo Bader
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分析服务

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