RNAseq of mESC expressing different epigenetic editing machineries

·发布 2023年10月1日

样本数

实验数

实验描述

PolyA RNAseq of mouse ESC carrying a stable integration of an epigenetic editing machinery, consisting of: dCas9-GCN4, individual effectors fused to scFV and gRNA against the Hbby locus. The system is induced by doxycycline addition to the culture media. Total RNA was isolated from cell pellets using NEB Monarch® Total RNA Miniprep Kit and libraries prepared using NEBNext RNA Ultra II kit (NEB E7770S, input: 350 ng of total RNA). Libraries were multiplexed and sequenced on a NextSeq 500 (Paired-End; read length 40). Each sample is present in two biological replicates.

样本属性

Organism

Mus musculus

Strain

FVB/NJ or mixed C57BL/6 and 129 mouse strain

Cell line

mESC

Cell type

embryonic stem cell

Genotype

dCas9-GCN4, DotL-CDscFV, dCas9-GCN4, Dnmt3a3L-CDscFV, dCas9-GCN4, GFP-scFV, dCas9-GCN4, p300-CDscFV, dCas9-GCN4, Prdm9-CDscFV, dCas9-GCN4, Kmt5C-FLscFV, dCas9-GCN4, G9a-CDscFV, dCas9-GCN4, Ezh2-FLscFV, dCas9-GCN4, Ring1b-CDscFV, dCas9-GCN4, Setd2-CDscFV

Genetic modification

stable integration of dCas9-GCN4, Dnmt3a3L-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, G9a-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, Prdm9-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, DotL-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, Ring1b-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, GFP-scFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, Setd2-CDscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, Ezh2-FLscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, Kmt5C-FLscFV and gRNA against Hbby locus, stable integration of dCas9-GCN4, p300-CDscFV and gRNA against Hbby locus

实验信息

登记号

E-MTAB-12101

实验类型

RNA-seq of coding RNA

物种

Mus musculus, Mus musculus

发布日期

2023年10月1日

提交者

Charles Girardot

外部链接

ArrayExpress 原始页

分析服务

RNAseq of mESC expressing different epigenetic editing machineries

联系地址

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