Mosaic perturbation of transcription factors in human retinal organoid development

·Released Feb. 24, 2023

Samples

Assays

References

Description

To begin to understand how TFs regulate retinal cell type identity in human tissues, we established a pooled loss of function (LOF) experiment based on the CROP-seq protocol in developed retinal organoids. We targeted five TFs (OTX2, NRL, CRX, VSX2, and PAX6) that are important for retinal development and expressed dynamically over the organoid developmental time course.

References

Multimodal spatiotemporal phenotyping of human organoid development

Philipp Wahle, Giovanna Brancati, Christoph Harmel, Zhisong He, Gabriele Gut, Jacobo Sarabia del Castillo, Aline Santos, Qianhui Yu, Pascal Noser, Jonas Simon Fleck, Bruno Gjeta, Dinko Pavlinić, Simone Picelli, Max Hess, Gregor Schmidt, Tom Lummen, Yanyan Hou, Patricia Galliker, David Goldblum, Marton Balogh, Cameron S. Cowan, Hendrik Scholl, Botond Roska, Magdalena Renner, Lucas Pelkmans, Barbara Treutlein, J. Gray Camp

Sample Attributes

Organism

Homo sapiens

Developmental stage

embryo

Disease

normal

Organism part

skin

Growth condition

retinal organoid

Progenitor cell type

induced pluripotent stem cell

Cell type

retinal cell

Cell line

409b2-iCas9

Genotype

mixed lentiviral pool against OTX2, NRL, CRX, VSX2, and PAX6, mixed lentiviral pool against OTX2, NRL, CRX, VSX2, and PAX6 with guideRNA

Experiment Info

Accession

E-MTAB-12622

Type

RNA-seq of coding RNA from single cells

Organism

Homo sapiens, Homo sapiens

Released

Feb. 24, 2023

Submitter

Zhisong He、 Giovanna Brancati、 Barbara Treutlein、 J. Gray Camp

External Links

ArrayExpress source

Analysis Services

Mosaic perturbation of transcription factors in human retinal organoid development

Contact

WeChat Official Account