Human embryonic stem cells were differentiated to early Pax6 positive neural epithelial progenitor cells and were differentiated further in neurospheres. On indicated days (hESC: n=3, day 0: n=3, day 5 n=3, day 15: n=2, day 22: n=1 and day 25: n=1) cells were extracted, mRNA prepared and hybridized in triplicates to Illumina Sentrix HumanHT-12 BeadChip gene arrays. More experimental detail is available under Hoelting et al., Archives of Toxicology.
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