RNA-seq of adult cardiac fibroblasts (aCFs), aCFs treated with TNF-α and IL-4 (aCF+CKs), and aCF+CKs expressing CD90 and VCAM1 (aVCFs)

This experiment aims at comparing the transcriptomic profiles of tree subpopulations of adult human cardiac fibroblasts (aCFs) defined by their relative expression of CD90 and VCAM1. Human adult cardiac fibroblasts (aCFs) were acquired from a commercial supplier; aCF+CKs were produced by addition of TNF-α and IL-4 into the culture medium; aVCFs were isolated from aCF+CKs by MACS against CD90 and VCAM1. The RNA-seq procedure was outsourced to GENEWIZ (South Plainfield, NJ, USA), and subsequent analysis was performed in-house. Briefly, total mRNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and the library was constructed with the NEBNext Ultra RNA Library Prep Kit for Illumina. Sequencing was realized on an Illumina HiSeq 2500 System.