Gene expression profiling of RGC-32-knockdown versus control MCF-7 cells using microarray analysis

MCF-7 cells were transfected with shRNA targeting RGC-32 and a negative control. Extracting high-quality total RNA (RIN > 8.0 verified by Bioanalyzer) followed by biotin labeling.Hybridizing labeled cDNA to microarrays under standardized conditions.Scanning arrays to generate raw data files (.CEL/.IDAT).Processing data using RMA or quantile normalization followed by differential expression analysis with limma (thresholds: |log2FC|>1, adj.p<0.05).