RNA profiling of the dorsal root ganglia, spinal cord, and anterior cingulate cortex in mice with CFA-induced inflammatory pain

To investigate the molecular mechanisms underlying pain and emotion - related responses in the nervous system, this experiment focused on dorsal root ganglia (DRG), spinal cord (SC), and anterior cingulate cortex (ACC) tissues. Mice were divided into sham and complete Freund's adjuvant (CFA) - induced inflammatory pain groups. The experimental workflow involved: first, extracting total RNA from the tissues using the miRNeasy kit; then, depleting ribosomal RNA and constructing strand - specific libraries with Illumina kits; next, performing RNA sequencing on the Illumina Nova6000 platform; and finally, conducting bioinformatics analyses including read trimming, genome mapping, differential gene expression analysis, and functional enrichment (GO, KEGG) to identify molecular changes associated with pain and emotion across the regions.