Molecular insights into DDX3X–mRNA regulation in castration-resistant prostate cancer

RNA-immunoprecipitation sequencing (RIP-seq) was performed to identify the transcripts under the regulation of DEAD-box helicase 3 X-linked (DDX3X). The BPH1-derived Cancer Progression (BCaP) cell lines were used for RIP-seq. Input and RIP RNA from the androgen receptor low/negative castration-resistant prostate cancer (ARL/- CRPC) BCaPMT10 and non-tumorigenic BCaPNT1 cells were extracted using the RNease Mini Kit. Libraries were prepared using the KAPA RNA HyperPrep Kit and sequenced on an Illumina HiSeq 2500 platform.