K562 cells treated with 10ng/ml TNFα, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags

Both Live-cell imaging studies and scRNA-seq studies have shown multiple heterogeneous responses to the same stimulus in the same cells. Therefore as a first application we investigated if ChronoSeq could also reveal the heterogeneity in stimulus response; we applied ChronoSeq to profile inflammatory responses in K562 cells stimulated with TNF-α, to capture the rapid NF-κB signaling cascade. 10 minute interval between samples including a 1 minute sampling duration.