Enhancing reprogramming towards human expanded pluripotency

Engineered SOX17 (eSOX17) factors were used to replace Yamanaka factor SOX2 in induced expanded potential stem cell (EPSC) reprogramming. eSOX17 achieved more efficient reprogramming under both feeder and feeder-free conditions. We have compared the iEPSC cell lines generated during the reprogramming with the embryo-derived EPSC control and found a similar gene expression pattern.