Single-cell RNA-seq analysis of mouse tracheal epithelial cells from wild-type and Tppp3 knockout mice

Single-cell RNA-seq was performed on tracheal epithelial cells from wild-type and Tppp3 knockout mice using the 10x Genomics Chromium Single Cell 3′ v3 platform. Seven major epithelial cell types were identified, and no novel cell type was detected in Tppp3 KO trachea. Expression of cilia-related genes (Mcidas, Foxj1, Myb, Ccno, Rfx2, Rfx3) showed no significant changes, suggesting that differentiation of tracheal ciliated cells was not affected by Tppp3 loss.