mRNA-seq in JURKAT T-ALL cell line upon shRNA knockdown of circN4BP2L2

We performed mRNA sequencing upon the lentiviral shRNA knockdown of circN4BP2L2 in JURKAT T-ALL cell line compared to non-targeting shRNA transduced cells to characterize the effect of this circRNA on gene expression profile. The cells were collected for RNA isolation 5 days after lentiviral transduction. Libraries were prepared using the TruSeq Stranded mRNA kit (fragmentation at 90 °C for 2 minutes [doi:10.1016/j.ygeno.2021.10.018]). Sequencing was carried out on an Illumina NovaSeqX platform (2×150 bp paired-end, ~150 million read pairs per sample). This work has been supported by National Science Centre, Poland (OPUS 2022/47/B/NZ5/00663).