Single-cell RNA sequencing reveals immune microenvironment changes induced by Fe-Shikonin treatment in SCC7 tumors

Single-cell RNA sequencing was performed on tumor tissues obtained from SCC7-bearing C3H mice to investigate the effects of Fe-Shikonin treatment on the tumor immune microenvironment. Tumor samples were collected from a phosphate-buffered saline (PBS)-treated control group and a Fe-Shikonin-treated group. In the deposited dataset, files designated as “CON1” correspond to tumor samples from the PBS control group, whereas files designated as “H1” correspond to tumor samples from the Fe-Shikonin treatment group. Single-cell libraries were constructed using the DNBSEQ-TaiM 4RS instrument (MGI) and DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) kit (Qingdao MGI Tech Co., Ltd).All libraries were sequenced on the DNBSEQ-T7RS platform (MGI) with paired-end reads.