Single-cell RNA-seq analysis of murine and human mesenchymal stem/stromal cells reveals macrophage-like phenotypic plasticity

This dataset contains single-cell RNA-seq profiles generated to investigate the phenotypic plasticity of mesenchymal stem/stromal cells (MSCs), with a focus on freshly isolated murine CD45-, TER119-, Sca-1+, PDGFRα+ (PαS) cells. Following transplantation into irradiated recipients, a subset of PαS cells acquired macrophage-like features, expressing CD45, CD68, and CSF1R/CD115. In vitro culture of PαS cells in macrophage differentiation media induced an M2-like signature (CD45, CD68, CD206) via CSF1R-dependent pathways. The dataset includes murine MSC samples from the C57/BL6 strain. The sequencing data support the identification of macrophage-like transcriptional clusters within the PαS population.