snRNA-seq of the developing chicken ovaries from E17-E20

This study used droplet-based snRNA-seq and joint snRNA-seq and snATAC-seq multiome to profile the gene expression of single nuclei in developing chicken ovaries across three timepoints, from embryonic day (E) 17 to E20. Fertilized chicken eggs (Gallus gallus) were purchased from Granja Santa Isabel and incubated at 37.5 °C in a humidified atmosphere until the required developmental stage. The day when eggs were incubated was considered E0. Single-cell barcoding and library preparation were performed using Chromium Single Cell 3' Reagent Kits (v3 chemistry) for snRNA-seq only and Chromium Single Cell Multiome ATAC + Gene Expression Reagent kits (v1) for multiome experiments with the Chromium Controller instrument (10x Genomics). Sequencing of the libraries was carried out on the Illumina NextSeq 500/550 and initial data processing was performed using Cellranger.