Transcription profiling by array of human colon cancer cell line SW480R with or without siRNA knockdown of transcription factor PROX1

·发布 2014年9月18日 ·更新 2014年9月11日

样本数

实验数

芯片平台

相关文献

实验描述

SW480R is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.SW480R cells are PROX1 positive. We suppressed through siRNA transfection the expression of PROX1 protein or non-targeting siRNA, using two different siRNA. Afterwards we compared the transcriptional program of the SW480-PROX1si and SW480R-CTRsi cells growing in monolayer in vitro.

参考文献

Cancer-specific target of Wnt PROX1 plays a a key role in the adaptation of metastatic colon cancer cells

Simone Ragusa, Jianpin Cheng, Konstantin Ivanov, Nadine Zangger, Jeremiah Bernier-Latmani, Stavros Milatos, Jean-Marc Joseph, Stefan Tercier, Hanifa Bouzourene, Frederick Bosman, Igor Letovanec, Penelope Andreux, Giancarlo Marra, Michel Gonzales, Mauro Delorenzi and Tatiana V. Petrova

芯片平台

A-AFFY-141

Affymetrix GeneChip Human Gene 1.0 ST Array [HuGene-1_0-st-v1]（6 例）

样本属性

cell line

SW480R

cell type

colon cancer cell

organism

Homo Sapiens

provider

ATCC

RNA interference

non-targeting negative control siRNA, PROX1 knock-down

实验信息

登记号

E-MTAB-2021

实验类型

transcription profiling by array

物种

Homo Sapiens

发布日期

2014年9月18日

更新日期

2014年9月11日

提交者

Nadine Zangger

外部链接

ArrayExpress 原始页

分析服务

Transcription profiling by array of human colon cancer cell line SW480R with or without siRNA knockdown of transcription factor PROX1

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