Full gene expression of pancreatic rat β-cell - RIN-m line treated with Edem2 siRNA

Non-treated, scrambled siRNA (control) and downregulated Edem2 samples were subjected to full gene expression. Ambion® Silencer® Select Pre-designed for rat Edem2 specific small interfering (siRNA) (cat. n. 4390771) and control (siSCR) (cat. n. 4390843) were purchased from (ThermoFisher). Lipofectamine® 2000 was used for transfecting siRNA in Opti-MEM™ Medium according to the manufacturer’s instruction. Edem2 mRNA level at 72 hours post-transfection was suppressed to 7.7% in siRNA cells compared to scrambled cells. The aim of this was to see the expression different when Edem2 was suppressed in normal condition.