Calibrated total RNAseq (cRNA-seq) of E14_Scc1_AID mESCs mixed with Drosophila SG4 cells with and without a 6-hour auxin treatment

We performed calibrated total RNAseq on mESC (Scc1-AID) for both untreated and treated cells with a 6-hour auxin exposure to assess the effects of cohesin removal on gene expression. For calibration purposes 2x10ˆ6 Drosophila SG4 cells were spiked in.