Capture-C experiments on auxin treated control (E14-Tir) and Scc1-AID, Ring1b-AID, Ring1b-AID-Scc1-AID and CTCF-AID (with and without a 6-hour auxin treatment) cells

提交 2018年9月14日 ·发布 2020年1月23日 ·更新 2020年1月23日

样本数

实验数

相关文献

实验描述

The aim of the experiment was to gain a higher resolution for specific regions of interest to complement the Hi-C results. Capture-C experiments were performed on four-inducible degrons for Scc1, Ringb, Ring1b-Scc1 and CTCF, and Tir1 control, always in triplicates (three biological replicates per condition).

参考文献

Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells

James D.P. Rhodes, Angelika Feldmann, Benjam�n Hern�ndez-Rodr�guez, Noelia D�az, Jill M. Brown, Nadezda A. Fursova, Neil P. Blackledge, Praveen Prathapan, Paula Dobrinic, Miles K. Huseyin, Aleksander Szczurek, Kai Kruse, Kim A. Nasmyth, Veronica J. Buckle, Juan M. Vaquerizas, Robert J. Klose

Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells

样本属性

age

0 hour

cell line

ES-E14 cell

cell type

mouse embryonic stem cell

date of experiment

11.12.2018, 17.09.2018, 23.06.2019

genotype

E14_CTCF_AID, E14_Ring1KO_Ring1b_AID, E14_Ring1KO_Ring1b_AID_Scc1_AID, E14_Scc1_AID, E14_Tir1

organism

Mus musculus

stimulus

Auxin treatment for 6 hours, Nocodazole (100ng/ml) for 6 hours, Untreated

strain

1290la

实验信息

登记号

E-MTAB-7840

GEO 编号

ERP114634

实验类型

Capture-C

物种

Mus musculus

提交日期

2018年9月14日

发布日期

2020年1月23日

更新日期

2020年1月23日

提交者

Angelika Feldmann、 Robert J Klose、 Juan M. Vaquerizas

外部链接

ArrayExpress 原始页 NCBI GEO：ERP114634

分析服务

Capture-C experiments on auxin treated control (E14-Tir) and Scc1-AID, Ring1b-AID, Ring1b-AID-Scc1-AID and CTCF-AID (with and without a 6-hour auxin treatment) cells

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