Single cell genotyping of Myeloid Leukaemia of Down Syndrome cells

Single cells from ML-DS sample 186-2 (bone marrow) were analysed to determine whether mutations in JAK2 and CSF2RB ocurred in the same or different cells in this patient. Single live, CD19neg, CD3neg, CD45mid and CD117 positive cells were sorted into 96-well plates on the BD Fusion with antibodies as described above. Cord blood single cells were sorted similarly to serve as a control for the single cell Sequencing.