Transcription profiling by array of human HEK293 cells overexpressing SOX11

We have carried out a cell line-based overexpression study coupled with microarray analysis to characterise the set of genes regulated by SOX11 and therefore critical for neurodevelopmental processes important in health and disease. The HEK293 cells were transiently transfected by pDEST40-SOX11 or control plasmid (pDEST40).Cells were collected at 24 hours post-transfection.Total RNA from alll samples was extracted.Biotinylated cRNA was prepared using the Illumina total Prep RNA application Kits.Hybridization, washing and scanning were performed according to the Illumina BeadStation 500* manual (revision C)