Comparative genomic hybridization of 30 ovarian cancer cell lines reveals amplification of PVT1 contributes to the pathophysiology of ovarian and breast cancer

DNA from 30 cultured ovarian cancer cell lines was extracted with Promega kits and hybridize to arrays. Arrays comprised of 1860 BACs selected to include genes known to be involved in cancer pathogenesis and 400 BACs selected to tile across 13 Mbp at 3q26, 15 Mbp at 8q24, and 30 Mbp at 20q centered on regions of recurrent amplification associated with reduced survival duration.