MIR200BGene Detail-Gene Database-Genelibs

MIR200B (microRNA 200b)

symbol:: MIR200B
locus group:: non-coding RNA
location:: 1p36.33
gene_family:: MicroRNAs
alias symbol:: hsa-mir-200b
alias name:: None
entrez id:: 406984
ensembl gene id:: ENSG00000207730
ucsc gene id:: uc001acw.3
refseq accession:: NR_029639
hgnc_id:: HGNC:31579
approved reserved:: 2004-04-23

1p36.33

ChineseEnglish

MIR200B属于microRNA（miRNA）基因家族中的miR-200家族，该家族还包括MIR200A、MIR200C、MIR141和MIR429。这些miRNAs在调控上皮-间质转化（EMT）过程中发挥关键作用，主要通过抑制ZEB1和ZEB2等转录因子的表达来维持上皮细胞表型，从而抑制肿瘤的侵袭和转移。MIR200B的生物学功能主要体现在调控细胞分化、增殖和凋亡，尤其在癌症中具有双重作用，既能抑制肿瘤转移，又在某些情况下促进肿瘤生长。它的主要作用位点是通过与靶基因mRNA的3'非翻译区（3'UTR）结合，导致mRNA降解或翻译抑制。MIR200B的突变或表达异常可能影响其调控网络，例如突变可能导致其无法正常抑制ZEB1/ZEB2，从而促进EMT和肿瘤转移。该基因与多种癌症相关，如乳腺癌、卵巢癌和肺癌，其低表达通常与肿瘤侵袭性增强和不良预后相关。当MIR200B过表达时，可增强上皮细胞特性并抑制转移，但也可能在某些情况下促进原发肿瘤生长；而降低表达则会促进EMT，增加细胞迁移和侵袭能力。miR-200家族的共性在于它们共享相似的种子序列，主要靶向ZEB1/ZEB2等EMT相关基因，并在维持上皮细胞状态和抑制肿瘤转移中发挥协同作用。此外，MIR200B还参与调控炎症反应和免疫微环境，其表达水平可能影响肿瘤对治疗的敏感性。

微RNA（miRNAs）是短（20-24核苷酸）非编码由既影响稳定性和mRNA的翻译参与基因表达的转录后调控在多细胞生物体的RNA。的miRNA通过RNA聚合酶II转录为加盖并聚腺苷酸化初级转录物的部分（PRI-微RNA），可以是任一蛋白质编码或非编码。初级转录由酶Drosha核糖核酸酶III酶裂解以产生约70个核苷酸茎 - 环前体miRNA（前miRNA），这是由胞质Dicer的核糖核酸酶进一步切割以产生成熟miRNA和反义miRNA的星（miRNA的* ）产品。成熟miRNA掺入RNA诱导的沉默复合物（RISC），其通过不完全碱基配对识别靶mRNA与所述miRNA和最通常导致翻译抑制或去稳定化的靶mRNA。所述的RefSeq表示预测的微RNA茎环。 [由RefSeq的，2009年09月提供]

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

OMIM entry for MIR200B Wikipedia entry for MIR200B

Nucleotide sequence of MIR200B:[NCBI]

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SNP variants of MIR200B: Showing partial SNPs

rs9442386 rs72563729 rs72563730 rs77342103 rs80296038 rs111233725 rs113882248 rs114497437 rs115542311 rs140316058 rs143277733 rs143556005 rs148003751 rs185213533 rs369057542 rs369073699 rs369205810

Tissue expression of MIR200B: [UniProt]

Forward Primer

Forward Tm

Reverse Primer

Reverse Tm

Score

No data available

Subcellular localization of MIR200B (and its protein):

[UniProt] [GenomeNet]

plasma membrane
cytoplasm
extracellular
golgi
vesicle
cytoskeleton
endoplasmic reticulum
nucleus
endosome
lysosome
mitochondrion

Gene Ontology (GO) terms for MIR200B:

microRNAs potentially regulating MIR200B:

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Interacting Gene	Interaction	Source/Score

KEGG
Reactomepathway

Name
5206 MicroRNAs in cancer [PATH:hsa05206]

Name
Pre-NOTCH Expression and Processing
Pre-NOTCH Transcription and Translation
Signal Transduction
Signaling by NOTCH

Disease

Score

NofPmids

NofSnps

Source

Disease	Score	NofPmids	NofSnps	Source
Disease Progression	0.12	1	0	CTD_human
Neoplasm Invasiveness	0.12	1	0	CTD_human
Precancerous Conditions	0.12	1	0	CTD_human
Liver Neoplasms, Experimental	0.12	1	0	CTD_human
Mammary Neoplasms	0.12	2	0	CTD_human
Neoplastic Cell Transformation	0.12	1	0	CTD_human
Malignant neoplasm of breast	0.000814326	3	0	BeFree
Breast Carcinoma	0.000814326	3	0	BeFree
Neoplasm Metastasis	0.000814326	3	0	BeFree
Tumor Progression	0.000542884	2	0	BeFree

The Role of MicroRNA-200 Family in Gastrointestinal Cancers.

Li J, Pan J, Wen J, Wang C Curr Mol Med IF: 2.8 2026-03-18

Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars.

Zhou Renpeng, Zhang Qi, Zhang Yun, Fu Shibo, Wang Chen Exp Cell Res IF: 3.5 2016-05-24

Androgen receptor as a regulator of ZEB2 expression and its implications in epithelial-to-mesenchymal transition in prostate cancer.

Jacob Sheeba, Nayak S, Fernandes Gwendolyn, Barai R S, Menon S, Chaudhari U K, Kholkute S D, Sachdeva Geetanjali Endocr Relat Cancer IF: 4.4 2015-01-22

Study of microRNAs (miRNAs) that are predicted to target the autoantigens Ro/SSA and La/SSB in primary Sjögren's Syndrome.

Gourzi V C, Kapsogeorgou E K, Kyriakidis N C, Tzioufas A G Clin Exp Immunol IF: 3.9 2015-12-17

Molecular regulation of ovarian cancer cell invasion.

Sun Ningxia, Zhang Qing, Xu Chen, Zhao Qian, Ma Yan, Lu Xinmei, Wang Liang, Li Wen Tumour Biol IF: 3.650 2015-04-17

Methylsorb: a simple method for quantifying DNA methylation using DNA-gold affinity interactions.

Sina Abu Ali Ibn, Carrascosa Laura G, Palanisamy Ramkumar, Rauf Sakandar, Shiddiky Muhammad J A, Trau Matt Anal Chem IF: 7.3 2015-09-23

Activation of miR200 by c-Myb depends on ZEB1 expression and miR200 promoter methylation.

Pieraccioli Marco, Imbastari Francesca, Antonov Alexey, Melino Gerry, Raschellà Giuseppe Cell Cycle IF: 3.9 2014-03-05

Aberrant microRNA expression in endometrial carcinoma using formalin-fixed paraffin-embedded (FFPE) tissues.

Lee Taek Sang, Jeon Hye Won, Kim Yong Beom, Kim Young A, Kim Min A, Kang Soon Beom PLoS One IF: 2.6 2014-07-28

TGF-β1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3.

Tong Jun, Fu Yuxuan, Xu Xinyu, Fan Shuxin, Sun Hong, Liang Ya, Xu Kai, Yuan Zhilan, Ge Yingbin Invest Ophthalmol Vis Sci IF: 5.5 2014-05-20