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PMID: 10022921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new member of the Sin3 family of corepressors is essential for cell viability and required for retroelement propagation in fission yeast.

Molecular and cellular biology ·Vol. 19 ·No. 3 ·1999-03-00 ·Pages 2351-65

Dang VD, Benedik MJ, Ekwall K, Choi J, Allshire RC, Levin HL

Abstract

Tf1 is a long terminal repeat (LTR)-containing retrotransposon that propagates within the fission yeast Schizosaccharomyces pombe. LTR-retrotransposons possess significant similarity to retroviruses and therefore serve as retrovirus models. To determine what features of the host cell are important for the proliferation of this class of retroelements, we screened for mutations in host genes that reduced the transposition activity of Tf1. We report here the isolation and characterization of pst1(+), a gene required for Tf1 transposition. The predicted amino acid sequence of Pst1p possessed high sequence homology with the Sin3 family of proteins, known for their interaction with histone deacetylases. However, unlike the SIN3 gene of Saccharomyces cerevisiae, pst1(+) is essential for cell viability. Immunofluorescence microscopy indicated that Pst1p was localized in the nucleus. Consistent with the critical role previously reported for Sin3 proteins in the histone acetylation process, we found that the growth of the strain with the pst1-1 allele was supersensitive to the specific histone deacetylase inhibitor trichostatin A. However, our analysis of strains with the pst1-1 mutation was unable to detect any changes in the acetylation of specific lysines of histones H3 and H4 as measured in bulk chromatin. Interestingly, the pst1-1 mutant strain produced wild-type levels of Tf1-encoded proteins and cDNA, indicating that the defect in transposition occurred after reverse transcription. The results of immunofluorescence microscopy showed that the nuclear localization of the Tf1 capsid protein was disrupted in the strain with the pst1-1 mutation, indicating an important role of pst1(+) in modulating the nuclear import of Tf1 virus-like particles.

MeSH Terms
Amino Acid Sequence Enzyme Inhibitors/pharmacology Fungal Proteins/genetics,metabolism Hemagglutinins Histone Deacetylase Inhibitors Histone Deacetylases/metabolism Hydroxamic Acids/pharmacology Molecular Sequence Data Mutagenesis Repressor Proteins/genetics,metabolism Retroelements Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Transcription Factors/genetics,metabolism
Chemicals
Enzyme Inhibitors Fungal Proteins Hemagglutinins Histone Deacetylase Inhibitors Hydroxamic Acids Repressor Proteins Retroelements SIN3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors pst1 protein, S pombe trichostatin A Histone Deacetylases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dang V D
Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Benedik M J
Ekwall K
Choi J
Allshire R C
Levin H L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.3.2351
Published
1999-03-00
Pages
2351-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC84027
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.19.3.2351 PMC Full Text View journal details
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