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PMID: 10023776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

BLNK required for coupling Syk to PLC gamma 2 and Rac1-JNK in B cells.

Immunity ·Vol. 10 ·No. 1 ·1999-01-00 ·Pages 117-25

Ishiai M, Kurosaki M, Pappu R, Okawa K, Ronko I, Fu C, Shibata M, Iwamatsu A, Chan AC, Kurosaki T

Abstract

Signaling through the B cell receptor (BCR) is essential for B cell function and development. Despite the key role of Syk in BCR signaling, little is known about the mechanism by which Syk transmits downstream effectors. BLNK (B cell LiNKer protein), a substrate for Syk, is now shown to be essential in activating phospholipase C (PLC)gamma 2 and JNK. The BCR-induced PLC gamma 2 activation, but not the JNK activation, was restored by introduction of PLC gamma 2 membrane-associated form into BLNK-deficient B cells. As JNK activation requires both Rac1 and PLC gamma 2, our results suggest that BLNK regulates the Rac1-JNK pathway, in addition to modulating PLC gamma 2 localization.

MeSH Terms

Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals B-Lymphocytes/enzymology,metabolism Calcium Signaling Calcium-Calmodulin-Dependent Protein Kinases/metabolism Carrier Proteins/genetics,metabolism,physiology Cell Membrane/enzymology Chickens Enzyme Activation Enzyme Precursors/metabolism GTP-Binding Proteins/metabolism Humans Intracellular Signaling Peptides and Proteins Isoenzymes/biosynthesis,genetics,metabolism JNK Mitogen-Activated Protein Kinases Mice Mitogen-Activated Protein Kinases Molecular Sequence Data Phospholipase C gamma Phosphoproteins Phosphorylation Protein-Tyrosine Kinases/metabolism Receptors, Antigen, B-Cell/metabolism,physiology Recombinant Fusion Proteins/biosynthesis Syk Kinase Type C Phospholipases/biosynthesis,genetics,metabolism Tyrosine/metabolism rac GTP-Binding Proteins ras Proteins/metabolism

Chemicals

Adaptor Proteins, Signal Transducing B cell linker protein Carrier Proteins Enzyme Precursors Intracellular Signaling Peptides and Proteins Isoenzymes Phosphoproteins Receptors, Antigen, B-Cell Recombinant Fusion Proteins Tyrosine Protein-Tyrosine Kinases SYK protein, human Syk Kinase Syk protein, mouse Calcium-Calmodulin-Dependent Protein Kinases JNK Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinases Type C Phospholipases Phospholipase C gamma GTP-Binding Proteins rac GTP-Binding Proteins ras Proteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Ishiai M

Department of Molecular Genetics, Institute for Liver Research, Kansai Medical University, Moriguchi, Japan.

Kurosaki M

Pappu R

Okawa K

Ronko I

Fu C

Shibata M

Iwamatsu A

Chan A C

Kurosaki T

Article Info

Journal

Immunity

Abbr.

Immunity

ISSN

1074-7613

DOI

10.1016/s1074-7613(00)80012-6

Published

1999-01-00

Pages

117-25

Language

English

Region

United States

NLM ID

9432918

Subset

Grants

NIAID NIH HHS · R01AI42787 · United States

NCI NIH HHS · R01CA71516 · United States

Databases

GENBANK

AF089727

External Links

PubMed source DOI: 10.1016/s1074-7613(00)80012-6 View journal details

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BLNK required for coupling Syk to PLC gamma 2 and Rac1-JNK in B cells.

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