Nuclease cleavage of chromatin at 100-nucleotide pair intervals.

Nature ·Vol. 264 ·No. 5586 ·1976-12-09 ·Pages 517-22

Altenburger W, Hörz W, Zachau HG

Abstract

Digestion of mouse liver nuclei with DNase II leads to a novel cleavage pattern with a 100-nucleotide pair periodicity. From chromatin, this pattern or the standard 200-nucleotide pair repeat can be produced depending on the ionic conditions. The results are interpreted by assuming different conformational states of the nuclear material, including condensed and extended forms.

MeSH Terms

Animals Binding Sites Cell Nucleus/metabolism Chromatin/ultrastructure Deoxyribonucleases/metabolism Deoxyribonucleoproteins/analysis Histones/metabolism Liver/ultrastructure Mice Micrococcal Nuclease/metabolism Molecular Weight Nucleic Acid Conformation Osmolar Concentration Protein Conformation Structure-Activity Relationship

Chemicals

Chromatin Deoxyribonucleoproteins Histones Deoxyribonucleases Micrococcal Nuclease

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Altenburger W

Hörz W

Zachau H G

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/264517a0

Published

1976-12-09

Pages

517-22

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/264517a0 View journal details

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Nuclease cleavage of chromatin at 100-nucleotide pair intervals.

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