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PMID: 10072403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation.

The Plant cell ·Vol. 11 ·No. 3 ·1999-03-00 ·Pages 445-58

Sheldon CC, Burn JE, Perez PP, Metzger J, Edwards JA, Peacock WJ, Dennis ES

Abstract

A MADS box gene, FLF (for FLOWERING LOCUS F ), isolated from a late-flowering, T-DNA-tagged Arabidopsis mutant, is a semidominant gene encoding a repressor of flowering. The FLF gene appears to integrate the vernalization-dependent and autonomous flowering pathways because its expression is regulated by genes in both pathways. The level of FLF mRNA is downregulated by vernalization and by a decrease in genomic DNA methylation, which is consistent with our previous suggestion that vernalization acts to induce flowering through changes in gene activity that are mediated through a reduction in DNA methylation. The flf-1 mutant requires a greater than normal amount of an exogenous gibberellin (GA3) to decrease flowering time compared with the wild type or with vernalization-responsive late-flowering mutants, suggesting that the FLF gene product may block the promotion of flowering by GAs. FLF maps to a region on chromosome 5 near the FLOWERING LOCUS C gene, which is a semidominant repressor of flowering in late-flowering ecotypes of Arabidopsis.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins DNA Methylation DNA, Plant/metabolism DNA-Binding Proteins/genetics Down-Regulation Gene Expression Regulation, Plant Homeodomain Proteins/genetics MADS Domain Proteins Plant Proteins/genetics Polymorphism, Restriction Fragment Length RNA, Messenger/metabolism RNA, Plant/metabolism Repressor Proteins/genetics Transcription Factors/genetics
Chemicals
Arabidopsis Proteins DNA, Plant DNA-Binding Proteins FIE protein, Arabidopsis Homeodomain Proteins MADS Domain Proteins Plant Proteins RNA, Messenger RNA, Plant Repressor Proteins Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sheldon C C
Commonwealth Scientific and Industrial Research Organisation, Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia.
Burn J E
Perez P P
Metzger J
Edwards J A
Peacock W J
Dennis E S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.3.445
Published
1999-03-00
Pages
445-58
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144185
Subset
IM
Databases
GENBANK
AF116527, AF116528
External Links
PubMed source DOI: 10.1105/tpc.11.3.445 PMC Full Text View journal details
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