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PMID: 10079972 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Oxidative resistance of Na/K-ATPase.

Cellular and molecular neurobiology ·Vol. 19 ·No. 1 ·1999-02-00 ·Pages 133-40

Kurella EG, Tyulina OV, Boldyrev AA

Abstract

1. Oxidative modification of Na/K-ATPase from brain and kidney has been studied. Brain enzyme has been found to be more sensitive than kidney enzyme to inhibition by both H2O2 and NaOCl. 2. The inhibition of Na/K-ATPase correlates well with the decrease in a number of SH groups, suggesting that the latter belong mainly to ATPase protein and are essential for the enzyme activity. We suggest that the differences in the number, location, and accessibility of SH groups in Na/K-ATPase isozymes predict their oxidative stability. 3. The hydrophilic natural antioxidant carnosine, the hydrophobic natural antioxidant alpha-tocopherol, and the synthetic antioxidant ionol as well as the ferrous ion chelating agent deferoxamine were found to protect Na/K-ATPase from oxidation by different concentrations of H2O2. The data suggest that these antioxidants are effective due to their ability to neutralize or to prevent formation of hydroxyl radicals.

MeSH Terms
Animals Antioxidants/metabolism Brain/enzymology Cattle Dogs Hydrogen Peroxide/pharmacology Hypochlorous Acid/pharmacology Kidney/enzymology Oxidants/pharmacology Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors,metabolism Sulfhydryl Compounds/metabolism,physiology
Chemicals
Antioxidants Oxidants Sulfhydryl Compounds Hypochlorous Acid Hydrogen Peroxide Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kurella E G
Laboratory of Clinical Neurochemistry, Institute of Neurology, Russian Academy of Medical Sciences, Moscow, Russia.
Tyulina O V
Boldyrev A A
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Article Info
Journal
Cellular and molecular neurobiology
Abbr.
Cell Mol Neurobiol
ISSN
0272-4340
DOI
10.1023/a:1006976810642
Published
1999-02-00
Pages
133-40
Language
English
Region
United States
NLM ID
8200709
Subset
IM
Grants
FIC NIH HHS · TW-00080 · United States
External Links
PubMed source DOI: 10.1023/a:1006976810642 View journal details
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