The boundaries of the silenced HMR domain in Saccharomyces cerevisiae.
The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It is believed that insulator/boundary elements separate these domains. Here we report the identification and characterization of boundary elements that flank the transcriptionally repressed HMR locus in the yeast Saccharomyces cerevisiae. Deletion of these boundary elements led to the spread of silenced chromatin, whereas the ectopic insertion of these elements between a silencer and a promoter blocked the repressive effects of the silencer on that promoter at HMR and at telomeres. Sequence analysis indicated that the boundary element contained a TY1 LTR, and a tRNA gene and mutational analysis has implicated the Smc proteins, which encode structural components of chromosomes, in boundary element function.
31 references, click to expand
-
Genetic and molecular analysis of the gypsy chromatin insulator of Drosophila.Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9378-83 PMID: 8790337
-
Heterochromatin and gene regulation in Drosophila.Curr Opin Genet Dev. 1996 Apr;6(2):193-202 PMID: 8722176
-
Silencers and locus control regions: opposite sides of the same coin.Trends Biochem Sci. 1997 Apr;22(4):124-8 PMID: 9149531
-
GCR1-dependent transcriptional activation of yeast retrotransposon Ty2-917.Yeast. 1997 Aug;13(10):917-30 PMID: 9271107
-
Sir- and silencer-independent disruption of silencing in Saccharomyces by Sas10p.Genetics. 1998 Jun;149(2):903-14 PMID: 9611201
-
Structure, dynamics, and function of chromatin in vitro.Annu Rev Biophys Biomol Struct. 1998;27:285-327 PMID: 9646870
-
Chromatin boundaries: punctuating the genome.Curr Biol. 1998 Jul 16;8(15):R521-4 PMID: 9705921
-
The regulation of yeast mating-type chromatin structure by SIR: an action at a distance affecting both transcription and transposition.Cell. 1982 Sep;30(2):567-78 PMID: 6215985
-
Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer.Cell. 1985 May;41(1):41-8 PMID: 3888409
-
A position effect on the expression of a tRNA gene mediated by the SIR genes in Saccharomyces cerevisiae.Mol Cell Biol. 1986 Feb;6(2):494-501 PMID: 3023851
-
Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
-
A position-effect assay for boundaries of higher order chromosomal domains.Cell. 1991 Mar 8;64(5):941-50 PMID: 1848159
-
Interactions of retrotransposons with the host genome: the case of the gypsy element of Drosophila.Trends Genet. 1991 Mar;7(3):86-90 PMID: 1851585
-
Active genes in budding yeast display enhanced in vivo accessibility to foreign DNA methylases: a novel in vivo probe for chromatin structure of yeast.Genes Dev. 1992 Feb;6(2):186-96 PMID: 1737615
-
A group of scs elements function as domain boundaries in an enhancer-blocking assay.Mol Cell Biol. 1992 May;12(5):2424-31 PMID: 1569958
-
Disruption of a silencer domain by a retrotransposon.Genetics. 1992 Jul;131(3):519-29 PMID: 1321064
-
SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae.Genes Dev. 1992 Jul;6(7):1319-31 PMID: 1628834
-
DNA position-specific repression of transcription by a Drosophila zinc finger protein.Genes Dev. 1992 Oct;6(10):1865-73 PMID: 1327958
-
SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.Genetics. 1992 Oct;132(2):325-36 PMID: 1330823
-
The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects.EMBO J. 1993 Feb;12(2):435-42 PMID: 8382607
-
Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.Genes Dev. 1993 Jul;7(7A):1133-45 PMID: 8319906
-
tRNA genes as transcriptional repressor elements.Mol Cell Biol. 1994 Feb;14(2):1266-77 PMID: 8289806
-
Yeast retrotransposons and tRNAs.Trends Genet. 1993 Dec;9(12):421-7 PMID: 8122309
-
Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation.Nucleic Acids Res. 1994 Mar 25;22(6):999-1005 PMID: 8152932
-
Silencers and domains of generalized repression.Science. 1994 Jun 17;264(5166):1768-71 PMID: 8209257
-
The Saccharomyces cerevisiae SPT8 gene encodes a very acidic protein that is functionally related to SPT3 and TATA-binding protein.Genetics. 1994 Jul;137(3):647-57 PMID: 8088510
-
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.Cell. 1994 Nov 4;79(3):449-58 PMID: 7954811
-
DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.Cell. 1994 Nov 4;79(3):459-74 PMID: 7954812
-
From the chromosomal loops and the scaffold to the classic bands of metaphase chromosomes.Cold Spring Harb Symp Quant Biol. 1993;58:755-65 PMID: 7525148
-
Silencing and heritable domains of gene expression.Annu Rev Cell Dev Biol. 1995;11:519-48 PMID: 8689568
-
Mitotic chromosome condensation.Annu Rev Cell Dev Biol. 1996;12:305-33 PMID: 8970729
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
