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PMID: 101423 Published · ppublish English Journal Article

Determination of prostaglandin synthetase activity in rectal biopsy material and its significance in colonic disease.

Gut ·Vol. 19 ·No. 10 ·1978-10-00 ·Pages 875-7

Harris DW, Smith PR, Swan CH

Abstract

A method is described for determining prostaglandin synthetase activity in milligram amounts of tissue. The procedure is based on the conversion of 14C-arachidonic acid to prostaglandin E2 and F2alpha-like substances. High levels of prostaglandin synthetase activity occurred in the inflamed mucosa of patients with ulcerative colitis and fell during successful drug therapy, but it is not yet known whether the cause of the inflammation first involves increased PG synthetase activity, or whether inflammation caused increase of PG synthetase.

MeSH Terms
Colitis, Ulcerative/enzymology Colonic Diseases, Functional/enzymology Humans Intestinal Mucosa/enzymology Methods Prostaglandin-Endoperoxide Synthases/metabolism Rectum/enzymology
Chemicals
Prostaglandin-Endoperoxide Synthases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harris D W
Smith P R
Swan C H
References (12)
12 references, click to expand
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  9. Letter: Prostaglandins, ulcerative colitis, and sulphasalazine.
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    J Obstet Gynaecol Br Commonw. 1971 Apr;78(4):289-93 PMID: 5558311
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Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.19.10.875
Published
1978-10-00
Pages
875-7
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1412356
Subset
IM
External Links
PubMed source DOI: 10.1136/gut.19.10.875 PMC Full Text View journal details
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