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PMID: 101510 Published · ppublish English Journal Article

Composition of the fractions separated by polyacrylamide gel electrophoresis of the lipopolysaccharide of a marine bacterium.

Journal of bacteriology ·Vol. 136 ·No. 1 ·1978-10-00 ·Pages 158-67

DiRienzo JM, MacLeod RA

Abstract

The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been determined. Conditions optimum for the release of constituent monosaccharides by hydrolysis were established. Sugars were quantitated by gas-liquid chromatography of their alditol acetate derivatives. Lipid A was detected by gel electrophoresis and by the spectral shift obtained with a carbocyanin dye. A comparison of the molar ratios of the various fractions suggest that LPS III is an LPS molecule lacking an O-antigenic side chain, whereas LPS I and II are LPS molecules differing in side-chain composition. LPS I may be a mixture of two LPS species. In double immunodiffusion experiments using anti-whole-cell serum, LPS I and II showed a homologous cross-reaction with isolated whole-cell LPS. LPS III as well as lipid A, core, and side-chain fractions failed to give rise to precipitin lines.

MeSH Terms
Cell Wall/analysis Electrophoresis, Polyacrylamide Gel Immunodiffusion Lipid A/analysis Lipopolysaccharides/analysis Molecular Weight Polysaccharides, Bacterial/analysis Pseudomonas/analysis,ultrastructure
Chemicals
Lipid A Lipopolysaccharides Polysaccharides, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DiRienzo J M
MacLeod R A
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.1.158-167.1978
Published
1978-10-00
Pages
158-67
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218645
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.1.158-167.1978 PMC Full Text View journal details
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